The effect of storage on the platelet hemostatic activity
E. V. Roitman, I. M. Kolesnikova (Moscow, Russia)
Time: 17:15 - 18:15
Objective: Apheresis and storage of platelet concentrates (PCs) affected by the platelets activation and total functional capacity of these cells. We assume that after transfusion the prevalence of platelets with changed activity lead to worse quality of blood clot in vivo. The aim was the in vitro study of platelet-dependent clot properties as a function of storage time.
Methods: Fifty single-donor apheresis PCs were divided in two groups: group 1 - platelets were remained in autologous plasma (PCs-P; n=26); group 2 – platelets were resuspended in PAS (SSP+; Macopharma, France) which substituted up to 70 vol% of autoplasma (PCs-PAS; n=24). Storage conditions were equal. PCs samples were analyzed by modified thromboelastography, and by aggregometry, and for platelets count, pH, lactate, glucose, and other platelets parameters. The testing were carried out in the day of proceeding, after 24 hours, and at 3rd and 5th days of storage.
Results: Platelets count had not significantly different between PCs-P and PCs-PAS. Between PCs-P and PCs-PAS no significantly differences had for platelets count, glucose consumption and lactate production. Moreover pH was almost unchanged that indicated buffer conditions were good. During the storage platelets aggregability and adhesion had worsened independently PCs type. But in PCs-P such decline was more expressed a little bit. We found that clot demonstrated gradual reduction of elasticity and deformability in both PCs groups. In PCs-P platelets lost their meaning for clot properties from the third storage day. In PCs-PAS activated platelets had no impact to clot properties during full storage time.
Conclusion: Irrespective of the proceeding method platelets viability was saved during the first five days of storage. It can be assumed that using platelet additive solution for PCs preparation does not reduce this cells viability. Platelets apheresis and storage are accompanied by aggregation-and-adhesion activity depression. Total decline of clot quality including low elasticity and impaired deformability was found of during period in stored PCs. We assume that clot properties are forming at the day of proceeding. Therefore we suppose that effect PCs transfusion is related to successful of platelets activity recovery in vivo.